Table 3.
Real-time PCR results (Ct values in BbRT2 real-time PCR) for tissues of γ-interferon knockout mice after inoculation with Besnoitia tarandi bradyzoites obtained from Canadian caribou skin.
Tissue | Mouse ID (time after inoculation) |
Median Ct over all positive animals | ||||
---|---|---|---|---|---|---|
K283-1 (5 dpi) | K283-2 (5 dpi) | K280-2 (8 dpi) | K280-1 (9 dpi) | K281 (18 dpi) | ||
Brain | 22.1 | 27.1 | 27.1 | 22.9 | 22.9 | 22.9 |
Heart | 20.1 | 24.9 | 24.9 | 22.9 | 19.1 | 22.9 |
Lung | 13.6 | 19.3 | 19.3 | 15.8 | 17.0 | 17.0 |
Liver | 20.8 | 24.0 | 24.0 | N/Aa | 42.3 | 24.0 |
Kidney | 45.5 | 39.6 | 39.6 | 34.9 | 25.4 | 39.6 |
Spleen | 30.0 | 30.6 | 30.6 | N/A | N/A | 30.6 |
Skeletal muscle | 20.2 | 23.1 | 23.1 | 20.6 | 22.3 | 22.3 |
Skin | 29.1 | 27.2 | 27.2 | 34.1 | 21.8 | 27.2 |
N/A = No amplification.