Figure 1.

Isolation and Identification of Human Sertoli Cells from OA and SCOS Patients

(A) RT-PCR showed the transcripts of GDNF, GATA4, SOX9, WT1, BMP4, and SCF in the isolated cells. PCR with PBS but without cDNA served as a negative control. (B) Western blots showed the protein levels of BMP4, SCF, and GDNF in OA and SCOS Sertoli cells. (C–L) Immunofluorescence demonstrated the expression of SOX9 (C), GATA4 (D), WT1 (E), VIM (F), GDNF (G), OCLN (H), SCF (I), VASA (J), α-SMA (K), and CYP11A1 (L) in the isolated cells. Replacement of primary antibodies with PBS was used as a negative control (M). The cell nuclei were stained with DAPI. Scale bars, 5 μm (C–M).