Figure 3.

miR-202-3p Inhibits the Synthesis Function of Human Sertoli Cells

The mRNA levels of GDNF (A), SCF (B), BMP4 (C), FGF2 (D), CXCL12 (E), and EGF (F) at 72 hr after virus infection and puromycin screening. (G) Western blots demonstrated GDNF, SCF, BMP4, FGF2, and CXCL12 proteins in human Sertoli cells at 72 hr after virus infection and puromycin screening. Results of three independent assays were concluded in (H). β-actin served as loading control of proteins. (I and J) CCK-8 assay showed the growth curve of a human spermatogonial stem cell line that was cultured with culture medium collected daily from the four Sertoli cell strains with upregulation of miR-202-3p (I) and downregulation of miR-202-3p (J). (K) Western blot demonstrated the expression of PCNA in human spermatogonial stem cell line after 72 hr of culture with culture medium collected daily from the four Sertoli cell strains. Results of the pre-miR group were normalized to the normal ctrl group, and results of the pre-miR inhibitor group were normalized to the inhibitor ctrl group. *p < 0.05; **p < 0.01; ***p < 0.001.