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. 2018 Oct 25;14:1–19. doi: 10.1016/j.omtn.2018.10.012

Figure 4.

Figure 4

miR-202-3p Promotes the Apoptosis of Human Sertoli Cells

(A–D) Annexin V-APC/PI and flow cytometry analysis revealed apoptosis in human Sertoli cells at 72 hr in the normal ctrl group (A), pre-miR group (B), inhibitor ctrl group (C), and pre-miR inhibitor group (D) after virus infection and puromycin screening. Results of three independent assays were concluded in (E). A total of 10,000 cells were analyzed. (F–I) TUNEL assay revealed the percentages of TUNEL+ cells in the normal ctrl group (F), pre-miR group (G), inhibitor ctrl group (H), and pre-miR inhibitor group (I) in human Sertoli cells after virus infection and puromycin screening. Results of three independent assays were concluded in (J). (K) Western blots demonstrated cPARP, Bcl2, and Bax proteins in human Sertoli cells at 72 hr after virus infection and puromycin screening. Results of three independent assays were concluded in (L). β-actin served as loading control of proteins. Results of the pre-miR group were normalized to the normal ctrl group, and results of the pre-miR inhibitor group were normalized to the inhibitor ctrl group. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars, 10 μm (F–I).