Figure 7.

LRP6 Knockdown Promoted Apoptosis of Human Sertoli Cells

(A) Annexin-V and propidium iodide (PI) staining and flow cytometry showed the percentage of apoptosis in human Sertoli cells after LRP6 knockdown. Results of three independent assays were concluded in (B). A total of 10,000 cells were analyzed. (C) TUNEL assay revealed the percentages of TUNEL⁺ cells in human Sertoli cells transfected with control siRNA or LRP6 siRNA-3. Results of three independent assays were concluded in (D). (E) Western blots demonstrated cPARP, Bcl2, and Bax proteins in human Sertoli cells at 48 hr after transfection with control siRNA or LRP6 siRNA-3. Results of three independent assays were concluded in (F). β-actin served as loading control of proteins. **p < 0.01; ***p < 0.001. Scale bars, 10 μm (C).