Fig. 6.

Human cornea immunofluorescence, results shown from cornea 2 (A & D), cornea 4 (G), cornea 6 (B & C), cornea 7 (E, F, H–L). All corneal tissues showed the same results, except for a small expression of fibrillin-1 directly under Bowman's membrane within the anterior cornea of corneas 3 and 4 (results not shown). Fig. 6 A–C: Type VI collagen expressed within the trabecular meshwork and corneal stroma of all corneal tissue analysed. Fig. 6 D–F: Fibrillin-1 expression appeared to be increased within the trabecular meshwork and posterior peripheral cornea. Expression appeared to decrease travelling towards the central cornea. Fibrillin-1 expression showed similar results across all corneal tissue, however, in addition, expression presented in the anterior corneal stroma directly inferior to Bowman's membrane in 2 corneas taken from the same donor (corneas 3 and 4), this was not seen in any other corneal tissue analysed. Fig. 6 G–I: Elastin expression within all three corneal tissues presented within the trabecular meshwork and posterior peripheral cornea, appearing enhanced anterior to Descemet's membrane (DM). No elastin expression presented within the central or anterior corneal stroma. Fig 6 J–L: Dual labelling shows elastin (green) and fibrillin-1 (red) co-localising (orange) within the TM and peripheral cornea. Fibrillin-1 is found present without elastin within the central cornea. S: stroma; TM: trabecular meshwork; SC: Schlemm's canal; DM: Descemet's membrane. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)