REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Cox4	Böttinger et al., 2015	GR578-4
Rabbit polyclonal anti-mtHsp70	Böttinger et al., 2015	GR2055-KB
Rabbit polyclonal anti-Tim23	Böttinger et al., 2015	133-4
Rabbit polyclonal anti-Tim44	Böttinger et al., 2015	128-4
Rabbit polyclonal anti-Om14	Ellenrieder et al., 2016	GR3040-6
Rabbit polyclonal anti-Sam37	Ellenrieder et al., 2016	161-8
Rabbit polyclonal anti-Sam50	Ellenrieder et al., 2016	312-17
Rabbit polyclonal anti-Mim1	Ellenrieder et al., 2016	GR1837-5
Rabbit polyclonal anti-Om45	Ellenrieder et al., 2016	GR1311-4
Rabbit polyclonal anti-Por1	This paper	GR3621-5
Rabbit polyclonal anti-Hsp60	Böttinger et al., 2015	170 (60)
Rabbit polyclonal anti-Tom20	Ellenrieder et al., 2016	GR3225-7
Rabbit polyclonal anti-Tom22	Ellenrieder et al., 2016	GR3227-2
Rabbit polyclonal anti-Tom40	Ellenrieder et al., 2016	168-5
Rabbit polyclonal anti-Tom70	Ellenrieder et al., 2016	GR657-3
Anti-HA peptide, mouse monoclonal antibody, clone 12Ca5	Roche	RRID: AB_514505; Cat. 11583816001
Anti-Penta-His, mouse antibody	QIAGEN	RRID: AB_2619735; Cat. 34660
Goat Peroxidase-coupled anti-Rabbit IgG	Jackson ImmunoResearch Laboratories	RRID: AB2313567; Cat. #111-035-003
Goat Peroxidase-coupled anti-Mouse IgG	Sigma	RRID: AB_258167; Cat. A4416
Chemicals, Peptides, and Recombinant Proteins
L-[35S]-Methionine	PerkinElmer	Cat. #NEG009005MC
MitoTracker Deep Red	Invitrogen	Cat. M22426
Glutathione S-Sepharose 4B	GE Healthcare	Cat. 17075601
Anti-HA affinity matrix	Roche	Cat. #11815016001
Ni-NTA Agarose	QIAGEN	Cat. #30230
Critical Commercial Assays
TNT® Quick Coupled Reaction Mix	Promega	Cat. #L2080
Roti®-Quant Bradford reagent	Roth	Cat. #K015.3
KOD Hot Start Master Mix	Merck Millipore	Cat. #71842-3
RedTaq Polymerase PCR Master Mix (2x)	Genaxxon Bioscience	Cat. #M3029.0500
mMessage mMachine SP6 Transcription Kit	Thermo Fisher Scientific	Cat. #AM1340
MEGAclear Transcription Clean-Up Kit	Thermo Fisher Scientific	Cat. #AM1908
Experimental Models: Organisms/Strains
YPH499 (WT) MATa ura3-52 lys2-801_amber ade2-101_orchre trp1-Δ63 his3-Δ200 leu2-Δ1	Sikorski and Hieter, 1989	1501
YPH499 (WT) rho-	Becker et al., 2011	1519
YPH499 rho- pRS415-TOM40HA	Becker et al., 2011	3177
YPH499 rho- Tom22::HIS3 pRS415-TOM40HA	Becker et al., 2011	3178
YPH499 arg4::kanMX4	This paper	2799
YPH499 tom22:TOM22HIS arg4::kanMX4	This paper	3955
YPH499 tom20::URA3 pYep13-TOM22	Becker et al., 2011	1273
YPH499 tom70::HIS3MX6	Becker et al., 2011	1059
YPH499 rho-Tom22::HIS3	Becker et al., 2011	2298
BY4741 (WT) MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0	EUROSCARF	1354
BY4741 xdj1:: kanMX4	EUROSCARF	3868
BY4741 djp1::KanMX4	EUROSCARF	3870
BY4741 ydj1::KanMX4	EUROSCARF	3869
BY4741 xdj1:: XDJ1HA-HIS3MX6	This paper	4447
BY4741 pUG-XDJ1GFP	This paper	4444
BY4741 pRS416	This paper	5277
BY4741 xdj1::kanMX4 pRS416	This paper	5278
BY4741 xdj1::kanMX4 pRS416-XDJ1	This paper	5280
BY4741 xdj1::kanMX4 pRS416-XDJ1H37Q	This paper	5315
Oligonucleotides
See Table S2 for oligonucleotide sequences		N/A
Recombinant DNA
pGEM4Z-TOM40 (S.cerevisiae)	Pfanner/Becker Labs	1495
pGEM4Z-CYB2(167)-DHFR (S. cerevisiae)	Pfanner/Becker Labs	B03
pGEM4Z-CYB2(167)Δ47-65-DHFR (S. cerevisiae)	Pfanner/Becker Labs	B04
pGEM4Z-TIM10 (S. cerevisiae)	Pfanner/Becker Labs	1235
pGEM4Z-Su9-DHFR (Su9 (1-69, N. crassa)-DHFR (mouse))	Pfanner/Becker Labs	S02
pGEM5X2	GE Healthcare	Cat. GE28-9545-54
pGEX5X2-XDJ1 (S. cerevisiae)	This paper	2576
pGEX5X2-XDJ1H37Q (S. cerevisiae)	This paper	2577
pGEX5X2-DJP1 (S. cerevisiae)	This paper	2580
pGEX5X2-YDJ1 (S. cerevisiae)	This paper	2579
pUG35-XDJ-GFP1 (S. cerevisiae)	This paper	2573
pRS416	Christianson et al., 1992	X 25
pRS416-XDJ1 (S.cerevisiae)	This paper	3112
pRS416-XDJ1-H37Q (S. cerevisiae)	This paper	3113
pFA6a-3xHA-HIS3MX6 (S. cerevisiae)	Knop et al., 1999	1450; pYM2
pET19b-TOM20CDHIS10(S.cerevisiae)	Brix et al., 1997	1811
pET19b-TOM22CDHIS10(S.cerevisiae)	Brix et al., 1997	1054
pET19b-TOM70CDHIS10(S.cerevisiae)	Brix et al., 1997	1055
Software and Algorithms
ImageJ	National Institue of Health, USA	https://imagej.nih.gov/ij/
Multi Gauge v.3.2	FujiFilm	N/A
Cell P	Olympus	N/A