Fig. 2 .

Confirmation of shifting ploidy status by FISH and FC. (A) U3021MG passage (P)13 displayed the same amount of diploid and tetraploid population, whereas U3021MG P21 contained 27% more diploid cells when analyzed by FC. (B) Representative FISH images of U3021MG. (C) U3028MG P8 and P16 contained the almost same percentage of diploid and tetraploid population between both methods. (D) FISH of U3028MG. (E) FISH and FC data confirmed the presence of diploid, tetraploid, and pentaploid cells in U3088MG P8 with slight variation in the percentage of population between both techniques. In U3088MG P16, the tetraploid population was not found by FC, whereas by FISH analysis 12% of tetraploid cells were identified. (B, D, F) Representative FISH images of U3021MG, U3028MG, and U3088MG. (F) Representative FISH images U3088MG.