Skip to main content
. 2018 Feb 2;20(8):1055–1067. doi: 10.1093/neuonc/noy012

Fig. 4 .

Fig. 4

Modulation of GCH1 levels in GBM cells affects oxidative stress in vitro. (A) Relative ROS levels in BTICs isolated from D456 xenografts with GCH1 overexpression using Promega ROS-Glo assay (normalized to vector control). P-value shown with t-test comparison. (B) Relative ROS levels in BTICs isolated from GBM D456 and GBM157 with GCH1 knockdown using Promega ROS-Glo assay (normalized to vector control). P-value shown with t-test comparison. (C) In silico analyses showing correlations between GCH1 and mRNA expression of PARK7 in human glioma gene expression datasets. Spearman rho value for each correlation is shown. (D) Analyses by qRT-PCR and western blot showing expression of the oxidative stress sensing molecule PARK7. P-value shown with t-test comparison. (E) Representative immunohistochemical analyses showing expression of the antioxidant protein PARK7 in D456 xenografts with and without GCH1 overexpression. (F) Growth of D456 and JX14 with GBM cells GCH1 shRNA was partially rescued by PARK7 cDNA as measured with the Promega CellTiter-Glo 2.0 assay; P-value shown with t-test comparison. Error bars represent standard deviations.