Table 3.
Extraction recovery and matrix effect of pinosylvin in rat plasma, urine, feces, and various tissue homogenates (n=5).
| Bio-sample | Nominal concentrations (ng/mL) | Extraction recovery (%) | RSD (%) | Matrix effect (%) | RSD (%) |
|---|---|---|---|---|---|
| Plasma | 5.4 | 98.8±7.1 | 8 | 99.1±8.5 | 8.6 |
| 135 | 95.2±2.5 | 3 | 103.3±5.4 | 5.8 | |
| 540 | 99.5±3.7 | 4.2 | 114.8±3.3 | 3.2 | |
| Urine | 1350 | 91.6±8.0 | 9.8 | 110.3±10.6 | 10.8 |
| 5400 | 108.5±2.5 | 2.6 | 81.3±5.3 | 7.3 | |
| 54000 | 98.3±6.8 | 7.7 | 107.2±9.2 | 9.6 | |
| Feces | 270 | 83.3±7.3 | 9.7 | 107.8±7.7 | 8 |
| 2700 | 99±6.1 | 7 | 98.2±6.7 | 7.6 | |
| 5400 | 96.4±4.4 | 5.1 | 97.8±5.9 | 6.8 | |
| Heart | 540 | 89.8±5.1 | 6.3 | 103.7±6.8 | 7.3 |
| 5400 | 99.3±5.3 | 6 | 94.1±7.1 | 8.4 | |
| 27000 | 99.8±2.6 | 2.9 | 96.9±6.5 | 7.5 | |
| Liver | 2700 | 86.5±4.8 | 6.3 | 103.2±4.7 | 5.1 |
| 13500 | 83.4±5.5 | 7.4 | 88.4±7.9 | 10 | |
| 54000 | 87.1±3.8 | 4.9 | 89.3±5.5 | 6.9 | |
| Spleen | 270 | 91.1±7.3 | 9 | 89.7±7.9 | 9.8 |
| 5400 | 93.5±7.3 | 8.7 | 94.8±7.3 | 8.7 | |
| 13500 | 93.7±5.2 | 6.2 | 93.7±8 | 9.6 | |
| Lung | 540 | 98.4±6.4 | 7.3 | 88.5±5.3 | 6.8 |
| 2700 | 91.5±6.7 | 8.2 | 91.6±7.8 | 9.4 | |
| 13500 | 86±4 | 5.2 | 90.5±6.9 | 8.5 | |
| Kidney | 540 | 86.5±6.1 | 7 | 98.4±8 | 8.2 |
| 5400 | 99.6±4.8 | 4.9 | 101.8±6.2 | 6.1 | |
| 13500 | 90.5±6.4 | 7 | 92.6±9.2 | 9.9 | |
| Large intestine | 2700 | 96.5±8.3 | 9.6 | 107.1±9.4 | 9.8 |
| 13500 | 98.6±5 | 5.7 | 99±6.8 | 7.7 | |
| 54000 | 98.9±5 | 5.6 | 93.0±7.6 | 9.1 | |
| Small intestine | 5400 | 89.4±10.7 | 13.3 | 88.7±7.7 | 9.7 |
| 108000 | 88.6±6 | 7.5 | 89.4±8 | 10 | |
| 216000 | 92.9±7.5 | 9.1 | 83.2±1.3 | 1.7 | |
| Stomach | 13500 | 87.5±10.1 | 13 | 90.8±7.1 | 8.8 |
| 216000 | 89.9±7.7 | 9.6 | 94±11.4 | 13.5 | |
| 864000 | 86.3±9.2 | 12 | 92.6±9 | 10.8 |