Figure 1.

Establishment of siRNA-mediated gene-silencing in HeLa cells. Three different siRNAs against Nrx (siNrx A-C) as well as unspecific control siRNA (siCtrl) were used to establish a specific gene-silencing of Nrx. HeLa cells were transfected twice by electroporation, and 72 h after each transfection, cell extracts were prepared. The Nrx protein levels were analyzed by immunoblotting using specific antibodies against Nrx and actin (a). The quantification of the Western blot signals was performed using ImageJ and shows that siNrx C induced the most efficient knockdown of Nrx (b). Confirmation of the siRNA-mediated knockdown of Nrx in SH-SY5Y cells (n = 3) (c).