Table 2.
Potential Nrx targets identified by all three approaches. The table contains the 51 proteins that were identified in all three experiments, i.e., the differential thiol labeling in Nrx-depleted SH-SY5Y cells, and the intermediate trapping using SH-SY5Y and mouse brain extracts. The proteins are sorted alphabetically; full lists can be found in the supplementary material.
| Protein ID | Gene name | Protein name |
|---|---|---|
| P49588 | AARS | Alanine-tRNA ligase, cytoplasmic |
| P23526 | AHCY | Adenosylhomocysteinase |
| P49419 | ALDH7A1 | Alpha-aminoadipic semialdehyde dehydrogenase |
| P04075 | ALDOA | Fructose-bisphosphate aldolase A |
| P48444 | ARCN1 | Coatomer subunit delta |
| P54687 | BCAT1 | Branched-chain amino acid aminotransferase, cytosolic |
| P78371 | CCT2 | T-Complex protein 1 subunit beta |
| P50990 | CCT8 | T-Complex protein 1 subunit theta |
| P23528 | CFL1 | Cofilin-1 |
| Q00610 | CLTC | Clathrin heavy chain 1 |
| O94760 | DDAH1 | N(G),N(G)-Dimethylarginine dimethylaminohydrolase 1 |
| P26641 | EEF1G | Elongation factor 1-gamma |
| P13639 | EEF2 | Elongation factor 2 |
| P09104 | ENO2 | Gamma-enolase |
| P21333 | FLNA | Filamin-A |
| Q16658 | FSCN1 | Fascin |
| P04406 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase |
| P62873 | GNB1 | Guanine nucleotide-binding protein |
| P17174 | GOT1 | Aspartate aminotransferase, cytoplasmic |
| P00505 | GOT2 | Aspartate aminotransferase, mitochondrial |
| Q92769 | HDAC2 | Histone deacetylase 2 |
| Q99714 | HSD17B10 | 3-Hydroxyacyl-CoA dehydrogenase type-2 |
| P07900 | HSP90AA1 | Heat shock protein HSP 90-alpha |
| Q9NSE4 | IARS2 | Isoleucine-tRNA ligase, mitochondrial |
| P12268 | IMPDH2 | Inosine-5′-monophosphate dehydrogenase 2 |
| Q07866 | KLC1 | Kinesin light chain 1 |
| P28838 | LAP3 | Cytosol aminopeptidase |
| P55209 | NAP1L1 | Nucleosome assembly protein 1-like 1 |
| P12955 | PEPD | Xaa-Pro dipeptidase |
| P14618 | PKM | Pyruvate kinase |
| P62937 | PPIA | Peptidyl-prolyl cis-trans isomerase A |
| P53041 | PPP5C | Serine/threonine-protein phosphatase 5 |
| Q06830 | PRDX1 | Peroxiredoxin-1 |
| Q9H6Z4 | RANBP3 | Ran-binding protein 3 |
| P54136 | RARS | Arginine-tRNA ligase, cytoplasmic |
| P39023 | RPL3 | 60S ribosomal protein L3 |
| P36578 | RPL4 | 60S ribosomal protein L4 |
| P61247 | RPS3A | 40S ribosomal protein S3a |
| Q16181 | SEPT7 | Septin-7 |
| P37837 | TALDO1 | Transaldolase |
| P60174 | TPI1 | Triosephosphate isomerase |
| Q71U36 | TUBA1A | Tubulin alpha-1A chain |
| Q9BVA1 | TUBB2B | Tubulin beta-2B chain |
| Q16881 | TXNRD1 | Thioredoxin reductase 1, cytoplasmic |
| P62987 | UBA52 | Ubiquitin-60S ribosomal protein L40 |
| P61088 | UBE2N | Ubiquitin-conjugating enzyme E2 N |
| P45974 | USP5 | Ubiquitin carboxyl-terminal hydrolase 5 |
| Q99536 | VAT1 | Synaptic vesicle membrane protein VAT-1 homolog |
| Q96QK1 | VPS35 | Vacuolar protein sorting-associated protein 35 |
| P54577 | YARS | Tyrosine-tRNA ligase, cytoplasmic |
| P63104 | YWHAZ | 14-3-3 protein zeta/delta (protein kinase C inhibitor protein 1) |