Figure 4.

The JNK inhibitor SP600125 promotes the proliferation of high glucose-induced pancreatic cancer cells. (a) PANC-1 and CFPAC-1 cells cultured in LG and HG media for 24 h in the presence or absence of SP600125 (10 μM). Cells were subjected to immunoblot analyses for the expression of p-JNK, JNK, p-c-Jun, c-Jun, and GAPDH. (b) Western blotting analysis of Bax, Bcl-2, CDK2, cyclin D1, and p21 in PANC-1 and CFPAC-1 cells after treatment as described in (a). Data are presented as mean ± SEM (n = 3). ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001, compared with the LG group.