Fig. 5 .

Tissue-based CyCIF was used to profile, quantify, and generate spatial representations of tumor and immune cell populations in whole slide sections (A, hematoxylin and eosin; B, representative “dot-plot”). Each immune population was quantified in the PD-L1 expressing tumor epithelium, PD-L1 negative epithelium, and stroma (C, representative data from PCP-3). The density of immune cells was quantified in ACP (n = 3) (D) and PCP (n = 3) (E) (mean ± SEM). The percent of stromal immune cells (CD45+) with PD-L1 expression was quantified in ACP (n = 3) and PCP (n = 3) (F).