Figure 4.

Inhibition of cell growth signaling by small molecule inhibitor leads. LNCaP cells were treated with recombinant SEMA3C (0.5 µM) alone or in combination with increasing concentrations of small molecules (0, 2.5, 5, 10, and 20 µM) followed by detection of phospho-EGFR, HER2/ErbB2, SHC, and MAPK. D13 attenuated SEMA3C-induced phosphorylation of EGFR, HER2/ErbB2, SHC, and MAPK. D36 inhibited SEMA3C-induced phosphorylation of MAPK only. D90 attenuated SEMA3C-induced phosphorylation of HER2/ErbB2, p46 SHC, and MAPK. Negative control small molecule D7 inhibited phosphorylation of EGFR, HER2/ErbB2, and p46 SHC but only at high concentrations.