Table 4.
Quantity and quality measurements of nucleic acids
| Archival biopsies | RNA | DNA |
|---|---|---|
| FFPE tissue input | 10 μm | 10 μm |
| Number of extractions | 84 | 84 |
| Yield (ng/μl) | ||
| Minimum | 1.6 | 0.6 |
| Maximum | 30.9 | 12.5 |
| Median | 9.2 | 3.3 |
| IQR | 6.35 | 2.3 |
| A260/A280 | ||
| Minimum | 0.7 | 1.1 |
| Maximum | 1.9 | 5.3 |
| Median | 1.5 | 1.7 |
| IQR | 0.2 | 0.4 |
| RIN-value | ||
| Minimum | 1.0 | – |
| Maximum | 4.1 | – |
| Median | 2.5 | – |
| IQR | 0.18 | |
| CT -value RNA18S5 | ||
| Minimum | 22.0 | 24.6 |
| Maximum | 39.3 | 38.7 |
| Mean | 28.8 | – |
| SD | 3.1 | – |
| Median | – | 29.2 |
| IQR | – | 6.4 |
Quantity and quality measurements of nucleic acids isolated from archival FFPE biopsy tissues from the prostate tumor. SD Standard Deviation, IQR Inter Quartile Range