Fig. 6.

Use of m-FISH using combined probes to detect multiple pathogens in intestinal tissue from CD. Oligonucleotide m-FISH images of intestinal tissue sections from four CD patients (a RS3, b RS4, c RS5, and d RS6). A combined probes mixture including (2) EC647 (non-pathogenic E. coli strain K-12 18s probe labeled with AF647 fluorophore) and (3) MAP488 (MAP UCF4 IS900 AV1 probe labeled with AF488 fluorophore) were used in all tissue sections. (1) DAPI staining and (4) merged images between DAPI and the corresponding probe. White measurement bar located in B3 represents 20 μm. All m-FISH images were obtained using CSLM