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. 2018 Dec 5;49:119. doi: 10.1186/s13567-018-0614-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transcription and expression of recombinant pcDNA3.1-TsDNase II in the MLN and spleen of vaccinated mice. Left: Transcriptions of TsDNase II in spleen and MLN of vaccinated mice were analyzed by RT-PCR. TsDNase II amplified fragments were observed in spleen (lane 1) and MLN (lane 2) from TsDNase II-vaccinated mice, but neither in spleen (lane 3) and MLN (lane 4) from pcDNA3.1 control mice, nor in spleen (lane 5) and MLN (lane 6) from the PBS group. Right: IIFT with anti-TsDNase II serum show that the fluorescence staining was detected in MLN (A) and spleen (D) of vaccinated mice, but the section of MLN (B) and spleen (E) of vaccinated mice were probed by normal serum. No immunostaining in MLN (C) and spleen (F) of pcDNA3.1 control mice was detected by anti-rTsDNase II serum. Scale bar: 50 μm.