Fig. 3.

KS enhanced the exploitation of the central cellular metabolism compared to single lesions. a Central cellular metabolism alteration in NSCLC NCI-H1299 isogenic clones harbouring KS, K and S relative to WT. b Central cellular metabolism alteration in NSCLC NCI-H1299 harbouring co-occurring KS relative to single K or S lesions. Measured proteins and metabolites are labelled as color-coded rectangle and circle respectively. Colours correspond to the fold-change in abundance relative to the parental counterpart (WT): red, increase; blue, decrease; black, not measured; grey, measured but not statistically significant. Metabolites and enzymes were reported using standard abbreviation. c Extracellular lactate concentrations determined in cultured conditioned medium 48 h after cells seeding. Data are expressed as cell-normalized mmol/L ± SD, n: 3 biological replicates. Significant differences were computed by one-way ANOVA and Tukey’s multiple comparisons test (GraphPad Prism, V7.02) ***p-value< 0.001