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. 2018 Dec 4;37:300. doi: 10.1186/s13046-018-0969-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Tetraspanin 1 (TSPAN1) induces epithelial-mesenchymal transition (EMT) in cholangiocarcinoma (CCA). (a and b) Expression of E-cadherin, N-cadherin, and vimentin was evaluated using western blot and real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in indicated processed cells and their corresponding control group. (c) E-cadherin and vimentin protein expression and subcellular localization were determined using immunofluorescence (IF) in CCLP1 and HCCC9810 cells. Data are means ± SD of three independent experiments. *p < 0.05 and **p < 0.01