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. 2018 Dec 4;37:300. doi: 10.1186/s13046-018-0969-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Tetraspanin 1 (TSPAN1) interacts with Integrin α6β1. (a and b) Co-immunoprecipitation (IP) showed TSPAN1 complexed with integrin α6β1. (c) Co-localization of TSPAN1 and integrin α6 was confirmed using immunofluorescence (IF) staining in CCLP1 and HCCC9810 cells. (d and e) Western blot and real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) experiments showed that integrin α6 expression was higher in CCA cells than in the normal cells. Experiments were performed three times and data are means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001