Fig. 8.
CircFAT1 functions as miR-375 sponge to promote tumorigenesis in vivo. a–b Nude mice were injected with 5 × 106 143B stable cells. Tumors were dissected and photographed after 5 weeks. c This graph represents tumor volumes (v = ab2/2) on the days the mice were injected with control cells or cells transfected with circFAT1 short hairpin (sh)RNA or co-transfected with circFAT1 shRNA and miR-375 sponge (n = 6 per group). Data represents the mean ± SD (n = 6). d Average tumor weight in each group at the end of the experiment (day 35). Data represent the mean ± SD (n = 6). * P < 0.05. e Histological analysis of tumor tissues by hematoxylin and eosin staining. YAP, c-Myc, and Birc5 expression was examined by immunohistochemistry. Scale bars, 100 μm (f) Western blot analysis of YAP, c-Myc, and Birc5 in tumors from xenograft mice. g qRT-PCR analysis of YAP, c-Myc, and Birc5 expression in tumors from xenograft mice. h Schematic illustration of the circFAT1/miR-375/YAP axis