Fig. 9.

Identification of protein interacts with MeGRXC15 in cassava and Arabidopsis. a Autonomous transactivation analysis of MeGRXC15 in yeast. MeGRXC15mP₆₅G₆₅ indicate mutant in MeGRXC15 GSH binding site. b Analysis of interaction between MeGRXC15 mP₆₅G₆₅ and TGA factors by yeast two-hybrid system. c BiFC analysis of the interactions between MeGRXC15 and TGAs identified by yeast two-hybrid system in transiently transformed N. benthamiana leaves. Green fluorescence in nucleus was detected for interactions of MeGRXC15 with MeTGA074 and AtTGA2, respectively. As a negative control, co-expression of MeGRXC15:YN with free YC, and MeGRXC15:YC with free YN failed to reconstitute a fluorescent YFP chromophore. Expression of MeTGA074:GFP and AtTGA2:GFP in transiently transformed N. benthamiana as positive controls. d Phylogenic analysis of TGA factors from Arabidopsis and cassava. A neighbor-joining tree was constructed with MEGA5.0 software based on sequences alignment with ClustalX