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. 2018 Dec 5;14:107. doi: 10.1186/s13007-018-0374-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Scheme summarizing the main steps of the Lipid-Linked Oligosaccharide (LLO) extraction method used in this study to extract and purify the oligosaccharide released from LLO of microalgae. UP: upper phase; INTER: intermediate phase; LOW: lower phase; SPN: supernatant resulting from solvent extraction, Mix: mixture; S: supernatant resulting from centrifugation; MP: microsomal pellet