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. 2018 Dec 5;14:107. doi: 10.1186/s13007-018-0374-8

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Multistage tandem mass spectrometry analysis of the permethylated LLO-released oligosaccharide isolated from C. reinhardtii XTA mutant (a–c) and from wild-type (d–f). ESI-MSⁿ spectra with n = 2 (a and d), n = 3 (b and e and n = 4 (c and f) of the [M + 2Na]²⁺ m/z 1107.6 precursor ion of the permethylated Hex₈HexNAc₂ derivative isolated from either the C. reinhardtii XTA mutant or the wild-type cells. The precursor ion selected for the fragmentation analysis is shown with a diamond and its fragmentation pattern is proposed according to Prien et al. [40]. Black square: N-acetylglucosamine; grey circle: mannose, black circle: glucose. The fragment ions are labelled according to the nomenclature of Domon and Costello [41]