Fig. 8.

Knockdown of jumu in the fat body and hemocytes induces activation of the Toll pathway. a-d Immunostaining against Dorsal (green) shows that knocking down jumu in the fat body under the control of ppl-Gal4 causes the nuclear localization of Dorsal in the fat body but not in circulating hemocytes. e-f” Clones expressing jumu RNAi (GFP⁺) also cause increased nuclear enrichment of Dorsal in adjacent wild-type clones (GFP⁻) (f-f”). g-l Immunostaining against Dorsal (red) and Dif (red) shows that the circulating hemocytes of Hml > GFP > jumu RNAi display activated Dorsal/Dif in the nuclei (arrows) (h and j), and Dif deficiency can rescue the nuclear localization phenotype of Dorsal/Dif (k and l). m, n Immunostaining against L1 (red) shows that Dif deficiency can reduce the generation of lamellocytes (arrows) in Hml > GFP > jumu RNAi circulating hemocytes. Scale bars: 20 μm (a, b, e-f”); 10 μm (c, d, g-n)