Fig. 5.

Structurally similar regions of three alphaherpesvirus transcripts neighboring OriS. The ORF63 of VZV, similarly to the HSV-1 US1 has a two-exon-containing splice isoform, while akin with PRV’s US1 the three-exon-containing splice isoforms. Both VZV and PRV express non-overlapping non-coding RNAs in the proximity of OriS. The target-specific primers used to confirm the TSSs of VZV’s NTO2, NTO3, NTO4 are shown as green arrows