Table 5.

VBeta T cell signatures and Streptococcus pyogenes toxin gene profiles of patients with streptococcal toxic shock syndrome.

	Treatments		Immunological data			Microbiological data
Gender	Antitoxin antibiotic (Clindamycin)	IVIGa (Dose)	Vβ alterations of CD3+ T cells (%)b	Vβ alterations of CD3+ T cells (%)	Toxin suspected according to Vβ modification profile	Site(s) of isolation	emm-types	Toxin gene profile
			Measurement 1 [day post shock onset]	Measurement 2 [day post shock onset]
Fc	Yes	Yes (2 g/kg)	Vβ1 ↔ (4.6%); Vβ2 ↔ (5.8%); Vβ5.1 ↔ (5.6%); Vβ12 ↔ (1.5%); Vβ14 (5.6%) [Dd+3]	Vβ1 (5.6%); Vβ2 ↔ (9.22%); Vβ5.1 ↔ (4.24%); Vβ12 ↔ (1.32%); Vβ14 (5.1%) [Dd+8]	SPEAe or SPECf	Throat	89	speBg, speCh
Mi	Yes	No	Vβ1 ↔ (2.2%); Vβ2 ↔ (10.6%); Vβ5.1 ↔ (4.7%); Vβ12 (5.8%); Vβ14 (22.1%) [D+4]	NDj	SPEA	Pleural effusion	1	speAk speB
F	Yes	Yes (2 g/kg)	Vβ1 (8.2%); Vβ2 (11.8%); Vβ5.1 ↔ (3.4%); Vβ12 ↔ (1.9%); Vβ14 ↔ (4.4%) [D+3]	ND	SPEC	Blood	87	speB, speC
M	Yes	Yes (2 g/kg)	Vβ1 ↔ (2.3%); Vβ2 (19.1%); Vβ5.1 (3.3%); Vβ12 (2.3%); Vβ14 (15.8%) [D+3]	ND	SPEA	Pleural effusion, blood	1	speA, speB
M	Yes	Yes (1 g/kg)	Vβ1 ↔ (2.9%); Vβ2 (27.6%); Vβ5.1 (2.5%); Vβ12 ↔ (1.4%); Vβ14 (6.8%) [D+1]	ND	SPEA or SPECj	Pleural effusion, blood	12	speB, speC
F	Yes	Yes (NAl)	Vβ1 ↔ (2.5%); Vβ2 (15.4%); Vβ5.1 (3.4%); Vβ12 (3.6%); Vβ14 (17.7%) [D+4]	ND	SPEA	Pleural effusion	1	speA, speB
F	Yes	Yes (1 g/kg)	ND	ND	ND	Wound	28	speB, speC
M	Yes	Yes (2 g/kg)	ND	ND	ND	Throat	ND	ND
F	No	No	ND	ND	ND	Blood	ND	ND
F	No	No	ND	ND	ND	Pleural effusion	1	speA, speB, speC
M	Yes	No	ND	ND	ND	Blood	12	speB
F	Yes	No	ND	ND	ND	Pleural effusion, blood	1	speA, speB
M	Yes	No	ND	ND	ND	Tracheal secretions	ND	ND
F	Yes	No	ND	ND	ND	Pleural effusion, tracheal secretions	6	speB, speC
M	Yes	Yes (2 g/kg)	ND	NDl	ND	Pleural effusion	1	speA, speB, speC

^aIVIG, Intravenous Immunoglobulin.

^bVβ, alterations of CD3⁺ T cells (%): The expression of 24 main Vβ CD3+ T cells was determined by flow cytometry. Only the altered expression of Vβ CD3⁺ T cells is showed according the following normal adult ranges provided by the kit manufacturer: Vβ1: 2.18 to 4.88%Vβ2: 5.84 to 10.76%; Vβ5.1: 3.85 to 7.05%; Vβ12: 1.12 to 2.2%; Vβ14: 2.13 to 4.85%.

^cF, Female.

^dD, day.

^eSPEA, streptococcal pyrogenic exotoxin A.

^fSPEC, streptococcal pyrogenic exotoxin C.

^gspeB, gene encoding streptococcal pyrogenic exotoxin B.

^hspeC, gene encoding streptococcal pyrogenic exotoxin C.

ⁱM, male.

^jND, not determined.

^kspeA, gene encoding streptococcal pyrogenic exotoxin A.

^lNA, not available.

No specific other fundings for this study.