Figure 1.

1,25(OH)2D stimulates miR-181b-5p to inhibit CRH in the human placenta. (A) Term STB were treated with 1,25(OH)2D at concentrations as indicated for 24 h. mRNA and protein levels were assessed by RT-qPCR with normalization to GAPDH (top panel) or Western blot (bottom panel), respectively. Error bars were derived from three independent experiments. (B) Term STB were treated with miR-181b-5p anatagomirs (inhibitors) or VDR siRNA for 24 h followed by 1,25(OH)2D at final concentration of 10 nM for additional 24 h. mRNA and protein levels were assessed by RT-qPCR (top panel) or Western blot (bottom panel), respectively. Three independent experiments were performed.