Figure 4.

Validation of CRH targeted by miR-181b-5p with dual-luciferase assay. (A) Schematic presentation of DNA constructs as used in this assay. (B) Term STB were transiently transfected with WT constructs and miR-181b-5p mimics or non-specific miRNA mimics (NS) as indicated for 48 h followed by dual-luciferase assay. (C) Term STB was transiently transfected with mutant (Mut) constructs and miR-181b-5p mimics or non-specific miRNA mimics (NS) as indicated for 48 h followed by dual-luciferase assay. Three independent experiments were performed. *P < 0.01 (Student t test, 2-tailed).