Figure 1.

Recombinant Oncolytic Adenovirus KGHV500 Infected SGC7901 Cells

(A) SGC7901 cells were collected, fixed, dehydrated, paraffin embedded, sectioned, stained for H&E, and observed with a microscope at 400×. The cells exhibited the following significant atypia compared to normal cells: cell size varied, cell nuclei were larger and deep dyeing, and the nucleoplasmic ratio was higher. (B) The paraffin-embedded SGC7901 cells were sectioned, and anti-CD46 mAb was used to detect whether CD46 protein was expressed on SGC7901 cell surfaces. It was found that nearly all the cells expressed CD46 protein, which was the receptor for the recombinant oncolytic adenoviruses KGHV500 and KGHV400. (C–F) To further identify whether SGC7901 cells were infected by KGHV500 and KGHV400 adenoviruses, the cells were cocultured with KGHV500 or KGHV400 adenovirus at an MOI of 100 for approximately 48 hr, then observed by transmission electron microscopy. KGHV500 adenovirus particles were found in the SGC7901 cell nucleus (C) and cytoplasm (D), and KGHV400 adenovirus particles were found in the SGC7901 cell nucleus (E) and cytoplasm (F). (G) The infection efficiency of KGHV500 adenovirus on SGC7901 cells was detected by TCID₅₀, and an MOI of 100 was the best infection efficiency. (H) An MOI of 100 was also the best infection efficiency for KGHV400 adenovirus on SGC7901 cells.