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. 2018 Oct 23;11:90–101. doi: 10.1016/j.omto.2018.10.003

Figure 5.

Figure 5

Distribution of KGHV500 Adenovirus and Anti-p21-Ras scFv in Mouse Organs

(A) The expression of adenovirus hexon protein in all the mouse organs was detected by immunohistochemistry and observed with a microscope at 200× magnification. Adenovirus hexon was not expressed in the brain in either the CIK + KGHV500 group or the KGHV500 group due to the blood-brain barrier. There was no adenovirus hexon distributed in any of the mouse organs except the spleen in the CIK + KGHV500 group, whereas, in the KGHV500 group, adenovirus hexon was found in all the mouse organs, such as the heart, liver, spleen, and lungs. (B) To further confirm the expression of anti-p21-Ras scFv in the tumor and all the mouse organs, western blotting was used to detect it, and this showed that anti-p21-Ras scFv was expressed only in the tumor tissue and spleen in the CIK + KGHV500 group, but it was expressed in all of the mouse organs except the brain in the KGHV500 group.