Figure 6. Human C/EBPβ also confers resistance to inhibitory effect of IFN‐γ and IL‐4 on human iT _reg generation.

1. Flow cytometry of intracellular FOXP3 staining (right) or isotype control (left) in human CD4⁺CD25⁻CD45RA⁺ naïve T cells transduced with control retrovirus (Min) or retrovirus encoding hC/EBPβ and cultured for 2 days under conditions as indicated. Representative experiments are shown in the left panel and pooled data with mean ± s.e.m. are shown on the right. Histograms are gated for CD4⁺NGFR⁺. Statistical analysis was performed using unpaired two‐tailed t‐test (*P < 0.05; ns, not significant).
2. Flow cytometry of CFSE‐labeled human CD4⁺CD25⁻ T cells (responder) stimulated 4 days alone or together with NGFR⁺‐sorted human CD4⁺CD25⁻CD45RA⁺ naïve T cells (suppressor) transduced with control retrovirus (Min) or retrovirus encoding hC/EBPβ and cultured for 5 days in the presence of hTGF‐β, anti‐hIFN‐γ, and anti‐hIL‐4 Abs. Histograms are gated for CFSE⁺. The ratio of responder to suppressor is 1:0.2 (top) or 1:0.4(bottom). Data are representative of two independent experiments with consistent results.