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. 2018 Nov 19;19(12):e46943. doi: 10.15252/embr.201846943

Figure 2. βHBP fails to induce rapid oligomerization of TIFA.

Figure 2

  1. IL‐8 production after 6 h of treatment with digitonin and βHBP at indicated concentrations or digitonin and wt Shigella flexneri lysate in HeLa cells (Hoechst in blue, IL‐8 in red).
  2. Quantification of IL‐8 as shown in (A). Data correspond to the mean ± SD of three independent experiments.
  3. TIFA oligomerization at 6 h in TIFA‐GFP‐expressing HeLa cells treated as in (A).
  4. Quantification of TIFA oligomerization as shown in (C). Data correspond to the mean ± SD of three independent experiments.
  5. TIFA oligomerization at 30 min in cells treated as in (A). Data correspond to the mean ± SD of four independent experiments.
  6. Kinetics of TIFA‐GFP oligomerization upon treatment with digitonin alone or in combination with βHBP or Shigella flexneri lysate. Data correspond to the mean ± SEM of four independent experiments.
Data information: Statistical significance was assessed using one‐way ANOVA (B, D, E) or two‐way ANOVA (F) followed by Tukey's multiple comparisons test *P < 0.05, **P < 0.01, ****P < 0.0001, not significant (ns). (A, C): Scale bar, 20 μm.