Figure 1. Selected endosomal Rab RNAi screen identified Rab22A as a regulator of RE dynamics.

• A
  IFM images of KIF13A‐YFP‐transfected control and Rab‐knockdown HeLa cells. T_N: average tubule number (mean ± SEM, n = 3).
• B, C
  Graphs represent the measurement of KIF13A‐positive T_N (B) and T_L (C) in HeLa cells of Fig 1A (mean ± SEM). n = 3. n_c: total number of cells. n_t: total number of tubules. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ns = not significant (unpaired Student's t‐test).
• D
  IFM images of KIF13A‐YFP and mCherry‐Rab7A/11A/22A‐transfected HeLa cells.
• E
  Live cell imaging of GFP/mCherry‐Rab22A with respect to mCherry‐Rab11A or GFP‐Rab7A in HeLa cells. Magnified view of insets (at 0, 20, 40 s) are shown separately.
• F
  Pull‐down of different His‐KIF13A domains using HeLa cell lysate and then probed with indicated Rab proteins. The bead‐bound His‐KIF13A in each pull‐down was shown on the Coomassie‐stained gel. *, non‐specific bands. Note, part of this experiment was shown in Fig 5F.

Data information: In (A, D, E), arrowheads and arrows point to the KIF13A‐/Rab22A‐positive tubular REs and E/SEs, respectively. Scale bars: 10 μm.