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. 2018 Nov 27;8:413. doi: 10.3389/fcimb.2018.00413

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Copyright © 2018 Arredondo, Swearingen, Martinson, Steel, Dankwa, Harupa, Camargo, Betz, Vigdorovich, Oliver, Kangwanrangsan, Ishino, Sather, Mikolajczak, Vaughan, Torii, Moritz and Kappe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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P36^mCherry Expression in P. yoelii Sporozoites. (A) Live fluorescence microscopy of freshly isolated oocyst (top) or salivary gland (bottom) sporozoites show expression of P36^mCherry; transmitted light images are also shown. (B) Relative expression of P36^mCherry and P52 as compared to MTIP in oocyst and salivary gland sporozoites analyzed by western blot (1 M spz per lane). Membrane was incubated with α-mCherry (16D7) and donkey α-rat-HRP; stripped and incubated with α-PyP52 (13G10) and goat α-mouse-HRP; stripped and incubated with α-MTIP and goat α-rabbit-HRP as a loading control. Spz, P36^mCherry sporozoite lysate; C, uninfected midgut or salivary gland extracts used as control for nonspecific antibody binding; Oocyst spz from day 10 (Scale bar: 5 μm).