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. 2018 Nov 28;9:1368. doi: 10.3389/fphar.2018.01368

FIGURE 5.

FIGURE 5

IFN-β decreases LPS-induced ICAM-1/VCAM-1 expression through STAT3. (A–B) CF were transfected with scramble or 200 ng of si-STAT1, si-STAT2, and si-STAT3 for 8 h, serum-deprived for 24 h, pretreated with IFN-β (500 U/ml) for 1 h, and stimulated with LPS (1 μg/ml) for 8 h. (A) The upper panel shows a representative Western blot image, indicating expression levels of ICAM-1 and GAPDH (used as charge control). The graphical analysis is presented at the bottom of each panel. Error bars indicate the SD for three independent experiments. ∗∗∗p < 0.001 vs. scramble. +++p < 0.001 vs. scramble + LPS. ###p < 0.001 vs. scramble + IFN-β + LPS. (B) The upper panel shows a representative Western blot image, indicating expression levels of VCAM-1 and GAPDH (used as charge control). The graphical analysis is presented at the bottom of each panel. Error bars indicate the SD for three independent experiments. ∗∗∗p < 0.001 vs. scramble. +++p < 0.001 vs. scramble + LPS. ##p < 0.01 vs. scramble + IFN-β + LPS.