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. 2018 Nov 28;9:1391. doi: 10.3389/fphar.2018.01391

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Copyright © 2018 Fang, Ni, Dong, Li, Wu, Li, Lv, Huang, Lian and Ge.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of atrazine on fetal Leydig cell proliferation. Sections were dually stained with PCNA (the biomarker for a proliferating cell) and CYP11A1 (the biomarker for the fetal Leydig cell). Proliferation Leydig cell nuclear was labeled by red color, and all Leydig cell was labeled by green color. The representative photomicrographs of rat testis sections after the treatment of 0 (A), 25 (B), 50 (C), and 100 mg/kg (D) ATR. White arrows point to PCNA-positive/CYP11A1-positive cells. Scale bar = 50 μm. (E) The percentage of PCNA-positive/CYP11A1-positive cells of total fetal Leydig cells. Mean ± SE, n = 6. Identical letters designate no difference between two groups at P < 0.05.