FIG. 5.

Fold Change in mRNA expression in J774A.1 cells stimulated with IL-4. J774A.1 macrophage cells were grown to 80% confluence and induced to differentiate to M2 macrophage phenotype using 20 ng/ml IL-4 in the presence of vehicle (0.05% EtOH), 2.5 or 10 µM DDE, 10 µM NS-398. 24 h later, total RNA was isolated and analyzed for (A) Arg1, (B) Nos2, and (C) Ptgs2 by RT-qPCR. Comparisons were made between stimulated cells and unstimulated control. Statistically significant differences were determined with one-way ANOVA with Duncan’s Studentized range test for multiple comparisons. Data represent the mean ± SEM of three experiments. Treatment groups with the same letter are not significantly different (P < .05).