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. 2018 Oct 25;19(12):e45536. doi: 10.15252/embr.201745536

Figure EV1. Depiction of the SUnSET technique.

Figure EV1

(i) Puromycin is structurally similar to tyrosyl‐tRNA, but contains a non‐hydrolyzable amide bond where tyrosine is normally cleaved from the tRNA complex and incorporated into a polypeptide chain. (ii) Puromycin is added to a growing polypeptide chain and terminates elongation of the peptide chain synthesis.