Figure EV3. De‐repression of β‐catenin‐mediated translation repression is independent of transcription.

A10 cells were transfected with siRNAs targeting ctnnb1 (β‐catenin) or scrambled RNA (control) and 36 h later treated with either actinomycin D (0.5 μg/ml) or DMSO for 4 h. Before harvesting, the cells were pulsed with 0.5 μM puromycin for 15 min and subjected to Western blot analysis for detection of the puromycin incorporated peptides. β‐Catenin blots indicated efficacy of siRNA. Actin was used as a loading control, and p53 was used as a control for actinomycin D activity. The average puromycin/actin ratio is shown in the graphs to the right (n = 3). A one‐way ANOVA and Tukey post hoc test were performed, with P‐values indicated above the error bars.Source data are available online for this figure.