Figure 3. ONX 0914 but not PRN1126 inhibits LMP2.

1. Hydrolysis of fluorogenic substrates (Ac‐PAL‐AMC) for LMP2 activity of human (upper panel) or mouse (lower panel) 20S immunoproteasome at various concentrations of PRN1126 or ONX 0914. Data are presented as the means of fluorescence ± s.d. from quadruplicate assays. The experiments were repeated three times with similar results.
2. Altered electrophoretic mobility of IP subunits by covalent modification with ONX 0914. Ficoll‐enriched lymphocytes from C57BL/6 mice were treated with DMSO or 300 nM ONX 0914 for 2 h in vitro. SDS–PAGE and immunoblotting against indicated proteins were performed. Shown are representative Western blots out of three independent experiments with similar outcome.

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