Figure EV3. Electrophoretic mobility shifts of IP subunits with different inhibitors.

• A
  Ficoll‐enriched lymphocytes from C57BL/6 mice were treated with DMSO, 300 nM ONX 0914, 300 nM PRN1126, 300 nM LU‐001i, or 300 nM ML604440 for 2 h in vitro. SDS–PAGE and immunoblotting against indicated proteins were performed. Shown are representative Western blots out of two independent experiments with similar outcome.
• B–E
  Hydrolysis of the fluorogenic substrate Bz‐VGR‐AMC for trypsin‐like activity of human (B, C) or mouse (D, E) 20S constitutive proteasome (B, D) or immunoproteasome (C, E) at various concentrations of ML604440 and MG132. Data are presented as the means of fluorescence ± s.d. from quadruplicate assays. The experiments were repeated three times with similar results.

Source data are available online for this figure.