Table 1.
PCR Primers
| Gene | Symbol | Forward primer | Reverse primer |
|---|---|---|---|
| Adrenoceptor beta 3 | Adrb3 | TGC GCA CCT TAG GTC TCA TTA | AAG GCG GAG TTG GCA TAG C |
| Fatty acid transporter | CD36 | GCC AAG CTA TTG CGA CAT GA | AAG GCA TTG GCT GGA AGA AC |
| Carnitine palmitoyltransferase I a | Cpt1a | GTG ACG TTG GAC GAA TCG GA | TCG GTG GCC ATG ACA TAC TC |
| Carnitine palmitoyltransferase I b | Cpt1b | TGT CAC TTC TGT CGC CAC CT | CAC CTC ATA ACG CTG GCT TC |
| Diacylglycerol O‐acyltransferase | Dgat | GTG GCC TTA CTGG TTGA GTC | CTG GAT AGC TCAC AGC TTGC |
| Deiodinase type II | Dio2 | CTT CCT CCT AGA TGC CTA CAA AC | CGA GGC ATA ATT GTT ACC TGA TTC |
| Macrophage antigen F4/80 | F480 | CTT TGG CTA TGG GCT TCC AGT C | GCA AGG AGG ACA GAG TTT ATC GTG |
| Facilitated glucose transporter | Glut4 | CCC CCG ATA CCT CTA CAT CAT C | GCA TCA GAC ACA TCA GCC CAG |
| Glycerol‐3‐phosphate acyltransferase | Gpat | GGA ATA CAG CCT TGG CCG AT | CTC TGT GGC GTG CAG GAA TA |
| Monocyte chemoattractant protein‐1 | Mcp‐1 | GGC TCA GCC AGA TGC AGT TAAC | GCC TAC TCA TTG GGA TCA TCT TG |
| Pyruvate dehydrogenase acetyl‐transferase | Pdk4 | TTC ACA CCT TCA CCA CAT GC | AAA GGG CGG TTT TCT TGA TG |
| Pparγ coactivator 1 alpha—total | Pgc1α | CGG AAA TCA TAT CCA ACC AG | TGA GAA CCG CTA GCA AGT TTG |
| Pparγ coactivator 1 alpha—1a | Pgc1α‐1a | GGG ACA TGT GC AGC CAA GA | AAG AGG CTG GTC CTC ACC AA |
| Pparγ coactivator 1 alpha—1b | Pgc1α‐1b | GAC ATG GAT GTT GGG ATT GTC A | ACC AAC CAG AGC AGC ACA TTT |
| Peroxisome proliferator‐activated gamma | Pparγ2 | GCA TCA GGC TTC CAC TAT GGA | AAG GCA CTT CTG AAA CCG ACA |
| PR domain containing 16 | Prdm16 | CTT CTC CGA GAT CCG AAA CTT C | GAT CTC AGG CCG TTT GTC CAT |
| Retinoblastoma 1 | Rb1 | CTG GCC TGT GCT CTT GAA GTT | CCA CGG GAA GGA CAA ATC TGT |
| Regulator of calcineurin 1—exon 1 | Rcan1.1 | TAG ATG GAG GAG GTG GAT CTG C | TCC TTG TCA TAT GTT CTG AAG AGG G |
| Regulator of calcineurin 1—exon 4 | Rcan1.4 | CCC GTG AAA AAG CAG AAT GC | TCC TTG TCA TAT GTT CCT GAA GAG GG |
| Stearoyl‐coenzyme A desaturase | Scd1 | GCC GAG AAG CTG GTG ATG TT | ATA GAG ATG CGC GGA ACT GT |
| Sarcolipin | Sln | GCT CCT CTT CAG GAA GTG AAG | TGG CCC CTC AGT ATT GGT AGG |
| Tumor necrosis factor‐alpha | Tnf‐α | GTA CCT TGT CTA CTC CCA GGT TCT CT | GTG TGG GTG AGG AGC ACG TA |
| Uncoupling protein 1 | Ucp1 | GCC AAA GTC CGC CTT CAG AT | TGAT TTG CCT CTG AAT GCC C |
| Zinc finger protein 423 | Zfp423 | CCC CCT GAT GGG AAT AAT GC | GCA ATG CGC CTG TTG GA |