Skip to main content
. 2015 Apr 16;31(16):2668–2675. doi: 10.1093/bioinformatics/btv213

Fig. 5.

Fig. 5.

Comparison between Final Structural Reactivities (FSRs) derived by StructureFold (Ding et al., 2014) versus gel-based assay in which reactivity is read out as band intensity. a. In vivo DMS treatment was performed on 1 μg of total RNA and analyzed using 32P radiolabelled 25S rRNA-specific primer (lane 2). A control with no DMS treatment was performed in parallel (lane 1). Dideoxy sequencing was conducted using 1 µg of total RNA (lanes 3–6). The region of 25S rRNA analyzed is nucleotides 1217–1303. b. FSRs derived by StructureFold (red) compared to normalized gel-based assay intensities (blue) on 25S rRNA for nucleotides 1217–1303. R = 0.69. c. ROC curve based on FSRs derived by StructureFold versus gel-based reactivities on 25S rRNA (nucleotides 1217–1303). d–g. ROC curves based on FSRs derived by StructureFold versus gel-based reactivities on 18S rRNA: nucleotides 17–86 (d), 87–207 (e), 298–428 (f), panels d–f combined (g)