Fig. 5.
Phenotypic analysis of PFMAGO-VIGS in P. floridana. a, b NC and PFMAGO-VIGS plants in 28DAI stage. c, d NC and PFMAGO-VIGS plants in the fruiting stage. e, f Phenotype of shoot apex growth at 28DAI stage in NC and PFMAGO-VIGS plants. g, h Shoot apex of NC and PFMAGO-VIGS plants. i, j NC and PFMAGO-VIGS flowers. k, l Pollen viability in NC and PFMAGO-VIGS flowers, which was evaluated by I2–KI staining. m The expression of both PFMAGO1 and PFMAGO2. Total RNAs from different flowers and number (n) as indicated were subjected to qRT-PCR. WT wild type, NC negative control, VIGS-I, PFMAGO-VIGS flowers showing pollen abortion; VIGS-II, PFMAGO-VIGS flowers showing WT-like pollen development. Bars, 10 cm in a–h, 1 cm in i and j, and 100 µm in k, l. Significance relative to NC was evaluated by a two-tailed student’s t test, and double asterisks indicates significance at P < 0.01