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. 2016 May 20;152(2):323–339. doi: 10.1093/toxsci/kfw092

TABLE 1.

Summary of Assay Sets.

Source Assay endpoints Note References
ACEA
  • ACEA

  • ACEA_cytotoxicity

2 Real-time cell electronic sensing (Abassi et al., 2009; Rotroff et al., 2013)
Apredica
  • APR_up

  • APR_dn

  • APR_cytotoxicity

58 High-content cell imaging; Duplicate in up and down direction (Giuliano et al., 2005, 2006, 2010; Taylor and Giuliano 2005)
Attagene
  • ATG_TRANS

  • ATG_CIS

82 Multiplex transcription reporter (Martin et al., 2010; Romanov et al., 2008)
BioSeek
  • BSK_Cytotoxicity

  • BSK_down

  • BSK_up

174 Biologically multiplexed activity profiling (BioMAP); Duplicate in up and down direction (Berg et al., 2005, 2006, 2010; Houck et al., 2009; Kleinstreuer et al., 2014)
Tox21
  • Tox21_BLA_Agonist

  • Tox21_BLA_Antagonist

  • Tox21_BLA_Cytotoxicity

  • Tox21_LUC_Agonist

  • Tox21_LUC_Antagonist

55 Cell-based qHTS (Attene-Ramos et al., 2015; Hsu et al., 2014; Huang et al., 2011, 2014; Xia et al., 2008)
NovaScreen ADME
  • NVS_ADME

  • NVS_ADME_Activator

30 Cell-free HTS Cytochrome P-450 activity assays; Duplicate in activator direction (Knudsen et al., 2011; Sipes et al., 2013)
NovaScreen Enzyme
  • NVS_ENZ

  • NVS_ENZ_Activator

115 Cell-free HTS other enzyme activity assays; Duplicate in activator direction (Knudsen et al., 2011; Sipes et al., 2013)
NovaScreen GPCR
  • NVS_GPCR

77 Cell-free HTS G-protein coupled receptor assays (Knudsen et al., 2011; Sipes et al., 2013)
NovaScreen Ion Channel
  • NVS_IC

22 Cell-free HTS ion channel assays, including ligand-gated ion channels (Knudsen et al., 2011; Sipes et al., 2013)
NovaScreen Nuclear Receptor
  • NVS_NR

20 Cell-free HTS radioligand binding nuclear receptor assays (Knudsen et al., 2011; Sipes et al., 2013)
NovaScreen Transporter
  • NVS_TR

9 Cell-free HTS transporter activity assays (Knudsen et al., 2011; Sipes et al., 2013)
NovaScreen Other
  • NVS_MP

2 Cell-free HTS activity assays (Knudsen et al., 2011; Sipes et al., 2013)
Odyssey Thera
  • OT

17 Protein complementation (Bolt et al., 2015; Stossi et al., 2014)

The total number of assay endpoints is 815. The bulleted items in the first column are the suffixes used in the different assay sets. Information on individual assays is available from http://actor.epa.gov/dashboard, last accessed May 19, 2016.