TABLE 1.
Summary of Assay Sets.
Source | Assay endpoints | Note | References |
---|---|---|---|
ACEA
|
2 | Real-time cell electronic sensing | (Abassi et al., 2009; Rotroff et al., 2013) |
Apredica
|
58 | High-content cell imaging; Duplicate in up and down direction | (Giuliano et al., 2005, 2006, 2010; Taylor and Giuliano 2005) |
Attagene
|
82 | Multiplex transcription reporter | (Martin et al., 2010; Romanov et al., 2008) |
BioSeek
|
174 | Biologically multiplexed activity profiling (BioMAP); Duplicate in up and down direction | (Berg et al., 2005, 2006, 2010; Houck et al., 2009; Kleinstreuer et al., 2014) |
Tox21
|
55 | Cell-based qHTS | (Attene-Ramos et al., 2015; Hsu et al., 2014; Huang et al., 2011, 2014; Xia et al., 2008) |
NovaScreen ADME
|
30 | Cell-free HTS Cytochrome P-450 activity assays; Duplicate in activator direction | (Knudsen et al., 2011; Sipes et al., 2013) |
NovaScreen Enzyme
|
115 | Cell-free HTS other enzyme activity assays; Duplicate in activator direction | (Knudsen et al., 2011; Sipes et al., 2013) |
NovaScreen GPCR
|
77 | Cell-free HTS G-protein coupled receptor assays | (Knudsen et al., 2011; Sipes et al., 2013) |
NovaScreen Ion Channel
|
22 | Cell-free HTS ion channel assays, including ligand-gated ion channels | (Knudsen et al., 2011; Sipes et al., 2013) |
NovaScreen Nuclear Receptor
|
20 | Cell-free HTS radioligand binding nuclear receptor assays | (Knudsen et al., 2011; Sipes et al., 2013) |
NovaScreen Transporter
|
9 | Cell-free HTS transporter activity assays | (Knudsen et al., 2011; Sipes et al., 2013) |
NovaScreen Other
|
2 | Cell-free HTS activity assays | (Knudsen et al., 2011; Sipes et al., 2013) |
Odyssey Thera
|
17 | Protein complementation | (Bolt et al., 2015; Stossi et al., 2014) |
The total number of assay endpoints is 815. The bulleted items in the first column are the suffixes used in the different assay sets. Information on individual assays is available from http://actor.epa.gov/dashboard, last accessed May 19, 2016.