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. Author manuscript; available in PMC: 2018 Dec 5.
Published in final edited form as: Mol Nutr Food Res. 2018 Jun 12;62(13):e1800189. doi: 10.1002/mnfr.201800189

Figure 4.

Figure 4.

A miR-140/TLR4/palmitic acid molecular mechanism promotes NAFLD in the absence of miR-140. A) Western blotting demonstrating that miR-140 overexpression inhibits palmitic acid mediated NFκB signaling activation. B) Expression of miR-140 after generation of a stable overexpression cell line using lentiviral vector. C) The putative binding site of miR-140 in the TLR4 3′UTR was identified using targetscan.org. D) Western blotting demonstrating miR-140 degradation of known miR-140 target fibronectin and TLR4. E) Dual luciferase assay verifying the targeting of TLR4 by miR-140.