Figure 4.

Fluorescence micrographs of the BMSCs distributed in the multi-tubular conduit with a length of 4.2 cm after incubation for 7 days. The conduit was frozen sectioned along the direction perpendicular to the longitudinal axis at the (A) proximal, (B) center, and (C) distal positions, and along direction parallel to the longitudinal axis at the (D) center and (E) boundary areas, respectively. F-actins of the cells were stained with Alexa Fluor 555 phalloidin (red) while the cell nuclei were stained with DAPI (blue). The inserted images at the top right corners in (D) and (E) are the overlay of fluorescence micrograph and the corresponding bright-field image.