Figure 5.

Representative fluorescence micrographs of the derived cells in the middle regions of the small tubes that were located (A) at the center (tube 1) and (B, C) in the periphery at opposite sites (tube 2 and tube 3) of the multi-tubular conduit, respectively, after transdifferentiation in the conduit with a length of 4.2 cm. The small tubes were cut open, and then the S100 protein were stained with Alexa Fluor 488 anti-S100 (green) and the cell nuclei were stained with DAPI (blue), respectively. (D) Mean pixel intensity (MPI) analysis of S100 expression from the derived cells in the middle regions of the different small tubes. No significant differences of the MPI were found among different tubes (n = 3).